Chemistry

• Thyroid disruption test: XETA (OECD 248)
Evaluation of all the thyroid mechanisms common to vertebrates - amphibian embryo – XP-T90-716-1 standard.

• Estrogenic disruption test: REACTIV (OECD filing in progress)
Evaluation of all the estrogenic mechanisms common to vertebrates – fish embryo – XP-T90-716-2 standard

• Androgenic disruption test: RADAR (OECD evaluation in progress)
Evaluation of all the androgenic mechanisms common to vertebrates – fish embryo – XP-T90-716-2 standard

• YES/YAS (Yeast Estrogen Screen/Yeast Androgen Screen) test on Saccharomyces Cerevisiae yeast
Confirmation of a specific endocrine mechanism for the estrogen or androgen receptor alpha.

» More information on endocrine disruption

• Duckweed – Lemna minor – NF EN ISO 20079
Growth inhibition assay (chronic toxicity)

• Daphnia – Daphnia magna – NF EN ISO 6341 / OECD 202
Daphnia immobilisation assay after 24 hours / 48 hours (acute toxicity)

• Ceriodaphnia – Ceriodaphnia dubia – NF ISO 20665
Growth inhibition and reproduction inhibition assay (chronic toxicity)

• Freshwater algae – Pseudokirchneriella subcapitata – NF EN ISO 8692 / OECD 201
Algae growth inhibition assay (algae chronic toxicity)

• Rotifers – Brachionus calicyflorus – NF ISO 20666
Growth inhibition assay (chronic toxicity)

• Microtox – Vibrio fischeri – NF EN ISO 11348-3
Bacterial luminescence inhibition assay (acute toxicity)

• Coral cuttings – Seriatopora Hystrix - method developed in-house
Study of the retraction of polyps and whitening of cuttings (acute toxicity)

• Marine algae – Phaeodactylum tricornutum – NF EN ISO 10253
Growth inhibition assays (acute toxicity)

• Bivalves – Crassostrea gigas – NF ISO 17244
Study of embryonic development

• Sea urchins – Paracentrotus lividus – internal methods
Study of embryonic development and/or spermiotoxicity assay

• Shellfish – Artemia salina – adapted from FD ISO 14669
Determination of acute lethal toxicity to marine copepods (acute toxicity)

• Amphipods – Corophium arenarium – NF EN ISO 16712
Mortality assay (acute toxicity)

• Rotifers – Brachionus plicatilis – non-standardised method
Mortality assay (acute toxicity)

» More information on ecotoxicity

• Ready biodegradability OECD 301 A: Dissolved Organic Carbon die-away test

• Ready biodegradability OECD 301 B: Carbon dioxide release test

• Ready biodegradability OECD 301 D: Closed bottle test

• Ready biodegradability OECD 301 F: Oxygen consumption test

• Intrinsic biodegradability OECD 302 B: Zahn-Wellens test

• Intrinsic biodegradability OECD 302 C: Modified MITI test (II)

• Certification according to the NF EN 14995 standard for plastic materials

» More information on biodegradability